The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials , from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption , elucidating their binding affinity , fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy . with the help of the concerned the data , the analysis was done to prove whether it can be applied to the zinc detection , in other words , whether it can be used as a new fluorescence probe for zinc detection 本實驗首次選用在生物體內(nèi)與zn ~ ( 2 + )鍵合能力很突出的物質(zhì)? ?組氨酸和半胱氨酸,采用類似于多肽合成的方法,在其羧基或氨基分別嫁接上一個帶有標(biāo)記的基團,生成穩(wěn)定的共價鍵化合物;在此化合物中模擬生理濃度條件加入鋅離子,通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標(biāo)記基團是否產(chǎn)生影響,再結(jié)合有關(guān)數(shù)據(jù)分析其是否適合檢測鋅離子,即是否可能作為新的鋅離子熒光探針。